Several players were injured in the crash and two people died.
Officials in Cumberland County coach Kristina Quigley, 30, a Baltimore native, was flown to a nearby hospital but was pronounced dead shortly thereafter.
Quigley was six months pregnant and her unborn baby also died.
The bus driver, 61-year-old Anthony Guaetta, died at the scene.
The team was heading to a Saturday afternoon game at Millersville University in central Pennsylvania for its fourth game of the year.
Seton Hill officials says they will hold a memorial Mass on campus Sunday night.