The closure will take place from midnight to 5 a.m. ET on May 13.
The brief shutdown will allow work crews to perform bridge demolition work, state transportation officials said on Friday.
All northbound vehicles will be temporarily detoured onto eastbound White Marsh Boulevard (Route 43) to Philadelphia Road (Route 7), then onto westbound White Marsh Boulevard and back onto I-95.
The express toll lane project should be completed some time in 2014.